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cpn60 clone panel. Plasmid DNA containing the cloned cpn60 UT sequence from each of the samples described below is available to the phytoplasma research community upon request (www.addgene.org). In all cases except O2L and SF1, amplicon was generated using the phytoplasma cpn60 UT primers described on the “primer sets” tab. |
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Clone Name |
cpn60 UT Classification | cpn60 UT GenBank Accession | cpnDB ID1 | 16S Classification | 16S GenBank Accession | Phytoplasma Species | Host | Country of Origin | Literature Reference | Plasmid Accession No.2 |
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O2L3 |
I-IA | KJ939998 | b27096 | 16SrI-A | MH279536 | ‘Ca. P. asteris’ | Onion | Canada (Saskatchewan) | (1) | 123000 |
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SF14 |
I-IB | KJ940013 | b27105 | 16SrI-B | MH279534 | ‘Ca. P. asteris’ | Flax | Canada (Saskatchewan) | (1) | 123001 |
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BbSP |
I-I(E/AI)AI | KU523402 | b27454 | 16SrI-E/AI5 | MH279523 (E); MH279522 (AI) | ‘Ca. P. asteris’ | Blueberry | Canada (Nova Scotia) | (2) | 123002 |
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CVB |
I-IF | KJ939995 | b27145 | 16SrI-F | MH279545 | ‘Ca. P. asteris’ | Periwinkle | Italy | (1) | 123003 |
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AY-Col |
I-IC | KJ939994 | b27144 | 16SrI-R | MH279535 | ‘Ca. P. asteris’ | Periwinkle | Italy | (1) | 123004 |
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R018 |
II-IC | MH279495 | b31187 | 16SrII-C | MH266698 | ‘Ca. P. aurantifolia’ | Lime | Iran | (3) | 123005 |
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TagTall |
IV-IC | MH922781 | b32834 | 16SrIV-C | MH922778 | ‘Ca. P. cocostanzaniae’ | Coconut | Tanzania | (3) | 123007 |
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Sab4 |
IV-IE | MH922782 | b32835 | 16SrIV-D | MH922779 | ‘Ca. P. palmae’ | Palm | USA (Florida) | (3) | 123008 |
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Palm A-DR |
IV-IE | MH922783 | b32836 | 16SrIV-E | MH922780 | ‘Ca. P. palmae’ | Coconut | Dominican Republic | (3) | 123009 |
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FD |
V-IC | KJ939992 | b27153 | 16SrV | NA6 | ‘Ca. P. ulmi’ | Grape | France | (1) | 123010 |
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PGY |
V-IC | KJ939991 | b27154 | 16SrV-C | MH279539 | ‘Ca. P. ulmi’ | Periwinkle | Germany | (1) | 123011 |
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RS |
V-IC | KJ939990 | b27155 | 16SrV-C | MH279544 | ‘Ca. P. rubi’ | Periwinkle | Germany | (1) | 123012 |
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Cr |
IX-IH | KJ939989 | b27158 | 16SrIX-A | NA7 | ‘Ca. P. phoenicium’ | Periwinkle | Cuba | (1) | 123014 |
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AP |
X-IA | KJ939977 | b27159 | 16SrX-A | MH279542 | ‘Ca. P. mali’ | Apple | Germany | (1) | 123016 |
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PYLR |
X-IC | KJ940010 | b27165 | 16SrX-C | MH279541 | ‘Ca. P. pyri’ | Peach | USA | (1) | 123017 |
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ESFY |
X-IF | KJ940007 | b27166 | 16SrX-F | MH279543 | ‘Ca. P. prunorum’ | Periwinkle | Italy | (1) | 123018 |
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SCWL |
XI-ID | MH279493 | b31184 | 16SrXI-D | KC295286 | ‘Ca. P. oryzae’ | Sugarcane | China | (3) | 123019 |
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BN44948 |
XII-IA | KJ939979 | b27168 | 16SrXII-A | MH279537 | ‘Ca. P. solani’ | Periwinkle | Germany | (1) | 123020 |
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SbGP-1 |
XIII-(A/I)I | KT444666 | b27178 | 16SrXIII-A/I8 | KT444665 (A); KT444664 (I) | ‘Ca. P.hispanicum’ | Strawberry | Mexico (Michoacan) | (4) | 123021 |
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PeruPBT |
XV-IB | MH279494 | b31186 | 16SrXV-B | KX810334 | ‘Ca. P. brasiliense’ | Papaya | Peru | (3, 5) | 123023 |
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1chaperonin database ID (http://cpndb.ca). |
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2accession number of plasmid at addgene (www.addgene.org). Plasmids are available to the scientific community via this repository. |
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3plasmid contains the 826 bp product of groEL F/H280p primers (1). Contains cpn60 UT and flanking cpn60 sequences |
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4plasmid contains the 1.4 kb product of groEL F/R primers (6). Contains cpn60 UT and flanking cpn60 sequences |
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5sample had F2nR2 sequence heterogeneity (7). The sequence MH279522 was suggested as a new subgroup, 16SrI-AI |
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6insufficient template volume remains to generate F2nR2 sequence; 16Sr group information provided by S. Malembic-Maher (personal communication) |
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7NA, not available. The 16S sequence generated from this sample was too short for iPhyclassifier (714 bp), but showed 100% id to GenBank KF941131, which is 16SrIX-A. |
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8sample had F2nR2 sequence heterogeneity (4). |
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